The objective of this course is to deliver comprehensive training on the fundamental techniques of both gas chromatography (GC) and liquid chromatography (HPLC). The course encompasses the key components of chromatography, including operating principles, applications, calibration methods, setup procedures, preventative maintenance, troubleshooting techniques, quantitative methods, and failure modes. Practical examples will be provided to illustrate these concepts. Additionally, the course will cover optimization strategies for column lengths, flows, temperatures, and pressure, accompanied by the necessary theoretical information for each aspect. The aim of this course is to enhance and advance the participants' skills and knowledge while providing them with practical expertise.

Course Objectives:

To impart participants with the fundamental techniques and knowledge of chromatography.
To familiarize participants with the tools and techniques used in chromatography analyses, cause and effect diagrams, calibration, and standard analysis methods.
To provide the skills, knowledge, and understanding of principles of chromatography analysis application.
To use routine maintenance.
To impart participants the operation technique.
To train participants to know how to change and install all chromatography components.
To be familiar with the latest analytical methods.

Who Should Attend?

The course is designed for chemists, lab technicians, chemical engineers, instrument engineers and lab supervisors/managers.

Course Outlines:

Fundamental chromatography technique
Chromatographic theory parameters
Chromatography retention process
Separation methods
Application of chromatography
Solid phase extraction method
Derivatization method
Standard operation method
Successful and safe operating procedure
Gas chromatography technique
Carrier gas and pressure regulator system
Split/splitless inlet system
Cool on-column inlet and programmed temperature vaporization inlet
Column configuration
Detector configuration
Maintenance and installation procedure for inject system, column and detectors
Liquid chromatography technique
Mobile phase solvent
Pumping system
Column and detector types
Operation method of HPLC
Overview of liquid chromatographic separation methods
Application of liquid chromatography
Ion chromatographic method
Size-exclusion chromatographic method
Supercritical fluid chromatography
Instrumentation and operating variables
Chromatography variables effect
Techniques to minimize peak broadening
Column condition and regeneration technique
Maintenance and installation procedure for inject system, column, pump and detectors
Troubleshooting and approaches to solve problems
Quantitative methods: calibration methods, external and internal standards, outliers test, determination of analyte concentration, standard addition method, error in quantitative analysis, confidence limits, detection limit, repeatability, reproducibility, and method validation.

Filters