This course aims to deliver comprehensive training on the fundamental techniques of gas chromatography (GC) and liquid chromatography (HPLC). Participants will gain valuable insights into the major components of chromatography, operating principles, applications, calibration methods, set-up procedures, preventative maintenance, troubleshooting techniques, quantitative methods, and failure modes. The course incorporates practical examples to enhance understanding.

Additionally, the course explores optimization strategies for column lengths, flows, temperatures, and pressure, providing participants with the necessary theoretical knowledge in each area. The primary objective is to enhance and advance the skills and knowledge of participants, enabling them to effectively operate chromatography techniques.

Course Objectives:

At the end of this Modern Laboratory Management Course, learners will be able to do:

To impart participants with the fundamental techniques and knowledge of chromatography.
To familiarize participants with the tools and techniques used in chromatography analyses, cause and effect diagrams, calibration and standard analysis methods.
To provide the skills, knowledge and understanding of principles of chromatography analysis application.
To use a routine maintenance.
To impart participants the operation technique.
To train participants to know how to change and install all chromatography components.
To be familiar with of the latest analytical methods.

Who Should Attend?

The course is designed for chemists, lab technicians, chemical engineers, instrument engineers and lab supervisors/managers

Course Outlines:

Fundamental chromatography technique
Chromatographic theory parameters
Chromatogra
phy retention process
Separation methods
Application of chromatography
Solid phase extraction method
Derivatization method
Standard operation method
Successful and safe operating procedure
Gas chromatography technique
Carrier gas and pressure regulator system
Split/split less inlet system
Cool on-column inlet and programmed temperature vaporization inlet
Column configuration
Detector configuration
Maintenance and installation procedure for inject system, column and detectors
Liquid chromatography technique
Mobile phase solvent
Pumping system
Column and detector types
Operation method of HPLC
Overview of liquid chromatographic separation methods
Application of liquid chromatography
Ion chromatographic method
Size-exclusion chromatographic method
Supercritical fluid chromatography
Instrumentation and operating variables
Chromatography variables effect
Techniques to minimize peak broadening
Column condition and regeneration technique
Maintenance and installation procedure for inject system, column, pump and detectors
Troubleshooting and approaches to solve problems
Quantitative methods: calibration methods, external and internal standards, outliers test, determination of analyte concentration, standard addition method, error in quantitative analysis, confidence limits, detection limit, repeatability, reproducibility and method validation

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