The objective of this course is to deliver comprehensive training on the fundamental techniques of gas chromatography (GC) and liquid chromatography (HPLC). The course encompasses various aspects, including the key components of chromatography systems, operating principles, applications, calibration methods, setup procedures, preventative maintenance, troubleshooting techniques, quantitative methods, and failure modes. Practical examples are provided to enhance understanding.

Furthermore, the course explores additional topics such as optimizing column lengths, flows, temperatures, and pressures. Theoretical information is provided to support the practical application of these optimization techniques. The ultimate goal of the course is to enhance participants' skills and knowledge, equipping them with the necessary expertise to proficiently operate chromatography techniques.

Course Objectives:

To impart participants with the fundamental techniques and knowledge of chromatography.
To familiarize participants with the tools and techniques used in chromatography analyses, cause and effect diagrams, calibration and standard analysis methods.
To provide the skills, knowledge and understanding of principles of chromatography analysis application.
To use a routine maintenance.
To impart participants the operation technique.
To train participants to know how to change and install all chromatography components.
To be familiar with of the latest analytical methods.

Who Should Attend?

The course is designed for chemists, lab technicians, chemical engineers, instrument engineers and lab supervisors/managers

Course Outlines:

Fundamental chromatography technique
Chromatographic theory parameters
Chromatogra
phy retention process
Separation methods
Application of chromatography
Solid phase extraction method
Derivatization method
Standard operation method
Successful and safe operating procedure
Gas chromatography technique
Carrier gas and pressure regulator system
Split/splitless inlet system
Cool on-column inlet and programmed temperature vaporization inlet
Column configuration
Detector configuration
Maintenance and installation procedure for inject system, column and detectors
Liquid chromatography technique
Mobile phase solvent
Pumping system
Column and detector types
Operation method of HPLC
Overview of liquid chromatographic separation methods
Application of liquid chromatography
Ion chromatographic method
Size-exclusion chromatographic method
Supercritical fluid chromatography
Instrumentation and operating variables
Chromatography variables effect
Techniques to minimize peak broadening
Column condition and regeneration technique
Maintenance and installation procedure for inject system, column, pump and detectors
Troubleshooting and approaches to solve problems
Quantitative methods: calibration methods, external and internal standards, outliers test, determination of analyte concentration, standard addition method, error in quantitative analysis, confidence limits, detection limit, repeatability, reproducibility and method validation

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